How Is It Made?

Organizes Living Human Skin Cells

  • Human keratinocytes and fibroblasts are derived from neonatal foreskins obtained for use under informed-consent guidelines1
  • The foreskin is decontaminated with antibiotics, antifungals, and an ethyl alcohol rinse
  • Production of cell stocks involves enzymatic digestion of the foreskin tissue and fibroblast/keratinocyte isolation1

...Into a Bilayered, Living Cell Based Treatment

  • Keratinocytes and fibroblasts are cultured and expanded under separate conditions1
  • Resulting cell stocks are frozen in cryopreservation vials and stored in master cell banks for later use1

Apligraf developed, manufactured and distributed by Organogenesis Inc.

References:
1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.

2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.