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Organizes Living Human Skin Cells
- Human keratinocytes and fibroblasts are derived from neonatal foreskins obtained for use under informed-consent guidelines1
- The foreskin is decontaminated with antibiotics, antifungals, and an ethyl alcohol rinse
- Production of cell stocks involves enzymatic digestion of the foreskin tissue and fibroblast/keratinocyte isolation1
...Into a Bilayered, Living Cell Therapy
- Keratinocytes and fibroblasts are cultured and expanded under separate conditions1
- Resulting cell stocks are frozen in cryopreservation vials and stored in master cell banks for later use1
Apligraf developed, manufactured and distributed by Organogenesis Inc.
References:
1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.
2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.