How Is It Made?

From Cells to Cell Therapy

Living human fibroblasts

• Removed from working cell bank, thawed, and expanded
• Placed on a semipermeable membrane along with bovine type I collagen and neutralizing medium1,2
• During 6-day incubation period:
  • Fibroblasts divide and multiply
  • Fibroblasts contract the collagen filaments1,2
  • Fibroblasts produce human matrix proteins
  • Dermal matrix forms and condenses1,2

Apligraf developed, manufactured and distributed by Organogenesis Inc.

References:
1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.

2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.