How Is It Made?

From Cells to Cell Therapy

Living human fibroblasts

• Removed from master cell bank, thawed, and expanded
• Seeded onto the contracted dermal matrix1,2
• During 4-day incubation period:
  • Keratinocytes attach to matrix1
  • Keratinocytes proliferate and differentiate
  • Epidermal layer forms

Apligraf developed, manufactured and distributed by Organogenesis Inc.

References:
1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.

2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.