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How Is It Made?

Step Two: Epidermal Layer Formation®

From Cells to Cell Therapy

Living human fibroblasts

  • Removed from master cell bank, thawed, and expanded
  • Seeded onto the contracted dermal matrix1,2
  • During 4-day incubation period:
    • Keratinocytes attach to matrix1
    • Keratinocytes proliferate and differentiate
    • Epidermal layer forms

Apligraf developed, manufactured and distributed by Organogenesis Inc.

1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.

2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.