Over a 20 day period

• Keratinocyte medium is replaced by a maintenance medium
• Cells continue to mature
• Distinct epidermal and dermal layers are present
• Developing bilayer is air-lifted (exposed to air)
• Incubated (air-liquid interface) for 10 days1,2
  • Enhances maturation of keratinocytes in epidermal layer
  • Produces a stratum corneum (cornification)

Apligraf developed, manufactured and distributed by Organogenesis Inc.

References:
1. Wilkins LM, Watson SR, Prosky SJ, et al. Development of a bilayered living skin construct for clinical applications. Biotechnol Bioeng. 1994;43747-756.

2. Parenteau NL, Nolte CM, Bilbo P, et al. Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991;45:245-251.